王宁，女，中国共产党员，博士，东北大学医学与生物信息工程学院，副教授，硕士生导师，中国生物工程学会青年工作委员会委员。

电子邮箱：wangning@bmie.neu.edu.cn

主要研究方向

1. 植物功能酶的挖掘与天然产物合成途径的搭建及解析

2. 基因组高效编辑、多靶点基因联合改造与代谢网络重构

3. 高通量筛选平台与底盘生产菌株的构建及改造

科研项目

1. 国家自然科学基金青年科学基金项目：定向进化外源蔗糖异构酶基因培育高异麦芽酮糖高粱新种质，2024-01至2026-12，30万元，项目负责人

2. 中国科学院，境内委托项目：基于多能互补系统的牧草品种培育及应用研究，2023-12至2024-12，47万元，项目负责人

3. 中国科学院，稳定支持基础研究领域青年团队项目：高产量高糖分甘蔗新品种培育，2022-07至2027-07，100万元，子课题主持

4. 中国科学院，中国科学院国合局战略咨询项目：“一带一路”沿线国家草牧业发展与海外粮仓建设的研究，2022-12至2024-12，40万元，项目骨干

5. 科学技术部，国家重点研发计划：课题四-非天然氨基酸生物合成及其适配底盘的设计及应用，2020-01至2024-12，536万元，子课题负责人

6. 国家自然科学基金委员会国际（地区）合作与交流项目：合成塑料讲解微生物的发掘及关键解聚酶的鉴定，2020-01至2023-12，500万元，项目骨干

代表性论著

1. Zeyu Liang; Chaoyong Huang; Yitian Li; Chao Yang; Ning Wang*; Xiaoyan Ma*; Yi-Xin Huo; A recombineering-based platform for high-throughput genomic editing in Escherichia coli, Applied and Environmental Microbiology, 2025, 91(7): e00193-25. IF5-year：4.5

2. Hao Guo#; Ning Wang#; Tingting Ding; Bo Zheng; Liwei Guo; Chaoyong Huang; Wuyuan Zhang; Lichao Sun; Xiaoyan Ma; Yi-xin Huo; A tRNA Modification-based strategy for Identifying amino acid Overproducers (AMINO), Metabolic Engineering, 2023, 78: 11-25 IF5-year：7.8

3. Ning Wang; Yi-Xin Huo; Using genome and transcriptome analysis to elucidate biosynthetic pathways, Current Opinion in Biotechnolgy, 2022, 76. IF5-year：8.3

4. Du Y. Song W., Ying Z., Wu S., Liu J., Wang N., Jin H., Qiao J. Genomic Analysis Based on Chromosome-Level Genome Assembly Reveals an Expansion of Terpene Biosynthesis of Azadirachta indica.Frontiers in Plant Science, 2022, 13:853861. IF5-year：5.7

5. Huiyan Wang; Ning Wang*; Yixin Huo; Multi-tissue transcriptome analysis using hybrid-sequencing reveals potential genes and biological pathways associated with azadirachtin A biosynthesis in neem (azadirachta indica), BMC Genomics, 2020, 21: 749. IF5-year：4.2

6. Ning Wang#; Kristine S. Bagdassarian#; Rebecca E. Doherty#; Johannes T. Kroon#; Katherine A. Connor; Xiao Y. Wang; Wei Wang; Ian H. Jermyn; Simon R. Turner, J. Peter Etchells; Organ-specific genetic interactions between paralogues of the PXY and ER receptor kinases enforce radial patterning in Arabidopsis vascular tissue, Development, 2019, 146(10): dev177105. IF5-year：4.6

7. 郝怀庆，张汝，卢呈，罗洪，李志刚，尚丽，王宁，刘智全，吴小园，景海春*。甜高粱育种研究进展及未来展望 [J/OL]。植物学报: 57(6):774-84 [2022-01-01] ISSN: 1674-3466中文核心。

8. Huiyan Wang, Hao Guo, Ning Wang*, Yi-Xin Huo* (2021) Toward the heterologous biosynthesis of plant natural products: gene discovery and characterization. ACS Synthetic Biology, 10 (11): 2784-95. DOI: 10.1021/acssynbio.1c00315. ISSN: 2161-5063. IF5-year：4.3

9. Tingting Ding, Chaoyong Huang, Zeyu Liang, Xiaoyan Ma*, Ning Wang* and Yi-Xin Huo (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.  Microbial Cell Factories, 19, 63. ISSN: 1475-2859.IF5-year：6.1

10. Jingge Wang, Chaoyong Huang, Kai Guo, Lianjie Ma, Xiangyu Meng, Ning Wang* and Yi-Xin Huo* (2020) Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA. Synthetic and Systems Biotechnology, 15; 5 (4): 333-342. ISSN: 2405-805X.IF5-year：4.5

11. Shaoya Li, Chen Zhang, Jingying LI, Lei Yan, Ning Wang, Lanqin Xia. Present and future prospects for wheat improvement through genome editing and advanced technologies.Plant Communications, 2021, 2(4):100211. IF5-year：11.8

12. Chaoyong Huang, Liwei Guo,Jingge Wang, Ning Wang*, Yi-Xin Huo.Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering.Applied Microbiology and Biotechnology, 2020, 104 (18):7943-56. IF5-year：5.1

13. Said Nawab, Ning Wang*, Xiaoyan Ma*, Yi-Xin Huo (2020) Genetic engineering of non-native hosts for 1-butanol production and its challenges:  a review.  Microbial Cell Factories, 19, 79. DOI: 10.1186/s12934-020-01337-w. ISSN: 1475-2859 IF5-year：6.1

14. Yi-Xin Huo, Bo Zheng, Ning Wang, Yunpeng Yang, Xinxin Liang, Xiaoyan Ma. Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers. Journal of Visualized Experiments, 148.（在线实验录制、受访）

15. Huan Yu#, Ning Wang#, Wenbo Huo, Yuhong Zhang, Wei Zhang, Yu Yang, Zhenya Chen, Yi-Xin Huo. (2019) Establishment of BmoR-Based biosensor to screen isobutanol overproducer. Microbial Cell Factories， 18（1）:30. IF5-year：6.1

16. Bo Zheng, Xiaoyan Ma, Ning Wang, Tingting Ding, Liwei Guo, Xiaorong Zhang, Yu Yang, Chun Li, YI-Xin Huo. (2018) Utilization of rare codon-rich markers for screening amino acid overproducers.Nature Communications, 9(1):3616 IF5-year：17.2

17. 郑博，王宁*，霍毅欣。基于转录和翻译调控的氨基酸高产菌株筛选及构建策略  [J/OL]。生物技术通报:  1-7 [2019-11-30] ISSN: 1002-5464.中文核心。

18. Huan Yu, Zhenya Chen, Ning Wang, Shenzhu Yu, Yajun Yan, Yi-Xin Huo.(2019)Engineering Transcription Factor BmoR for Screening Butanol Overproducers. Metabolic Engineering, 56:28-38.

19. Ning Wang, Xiaolin Wu, Lixia Ku, Yanhui Chen and Wei Wang* (2016).  Evaluation of three protein-extraction methods for proteome analysis of maize leaf midrib, a compound tissue rich in sclerenchyma cells.  Frontiers in Plant Science, 7 (108): 856. ISSN: 1664-462X. IF5-year：6.8

20. Ning Wang, Di Cao, Fangping Gong, Lixia Ku, Yanhui Chen * and Wei Wang*. (2015). Differences in properties and proteomes of the midribs contribute to the size of the leaf angle in two near-isogenic maize lines. Journal of Proteomics, 128: 113-122. ISSN: 1874-3919. IF5-year：3.5

21. Ning Wang, Lixia Ku, Yanhui Chen* and Wei Wang*. (2014). Comparative proteomic analysis of leaves between photoperiod-sensitive and photoperiod-insensitive maize inbred seedlings under long day treatments. Acta Physiologiae Plantarum, 37:1705. ISSN: 0137-5881. IF5-year：2.9

专利成果

1. 一种基于蔗糖致死基因(SacB)高效筛选蔗糖异构酶突变体的方法，专利号：CN202311436872.8

2. 一种能高效兼具特异性转化蔗糖的蔗糖异构酶突变体及应用，专利号：CN202311436873.2

3. 一种印楝素合成关键中间体的相关基因，专利号：CN202010862442.2

4. 一种印楝素合成前体的底盘生产菌株WHY-60，专利号：CN202010862804.8

5. 一种利用稀有密码子在翻译水平调控基因表达的方法，专利号：CN201910923897.8

6. 一种支链氨基酸高产菌株的筛选方法，专利号：CN201810710065.3

7. 一种极性氨基酸高产菌株的筛选方法，专利号：CN201810708895.2

8. 一种非极性氨基酸高产菌株的筛选方法，专利号：CN201810708595.4